Presynapse Formation Assay Using Presynapse Organizer Beads and “Neuron Ball” CultureShumaia Parvin 1, Renoma Takeda 1, Yukio Sasaki 1
1Functional Structure Biology Laboratory, Department of Medical Life Science, Yokohama City University Graduate School of Medical Life Science
Presynapse formation is a dynamic process including accumulation of synaptic proteins in proper order. In this method, presynapse formation is triggered by beads conjugated with a presynapse organizer protein on axonal sheets of “neuron ball” culture, so that accumulation of synaptic proteins is easy to be analyzed during presynapse formation.
